1351 Construction of a three-dimensional sensory stimulus evaluation model focusing on the cellular signal pathway though activation of TRPV1

Transient Receptor Potential (TRP) channels, which are molecular sensors for temperature detection, considered to play important roles in skin itch transmission and irritation. We focused on TRPV1 is expressed sensory neurons because our previous study showed that activated by ethanol or polyols used cosmetics the activity has good correlation with human However, only water-soluble substances can be evaluate activities a conventional method. In addition, it necessary design new evaluation system considering epithelial barrier functions since located lower layers of cells. study, we constructed co-culture HEK293 cells stably expressing (TRPV1 cells) three-dimensional model (3D model) having function. First, selected appropriate scaffold proteins keep cell proliferation function rate higher than those 3D model. Collagen-gel-embedded method maintained an original Next, screened indicators activation instead intracellular Ca2+levels it’s difficult detect this conventical indicator under system. As result, found MCP protein, member CC chemokine family proteins, released TRPV1-activity-dependent manner also excisional biopsy skin. These data suggest enable us water-insoluble substances.